(Adapted from //www.biologycorner.com/) A microscope is an instrument that magnifies an object so that it may be seen by the observer. Because cells are usually too small to see with the naked eye, a microscope is an essential tool in the field of biology. In addition to magnification, microscopes also provide resolution, which is the ability to distinguish two nearby objects as separate. A combination of magnification and resolution is necessary to clearly view specimens under the microscope. The light microscope bends a beam of light at the specimen using a series of lenses to provide a clear image of the specimen to the observer. In this lab, parts of the microscope will be reviewed. Students will learn the proper use and care of the microscope and observe samples from pond water.
Your microscope has 4 objective lenses: Scanning (4x), Low (10x), High (40x), and Oil Immersion (100x). In this lab, you will not use the oil immersion lens; it is for viewing microorganisms and requires technical instructions not covered in this procedure.
In addition to the objective lenses, the ocular lens (eyepiece) has a magnification. The total magnification is determined by multiplying the magnification of the ocular and objective lenses.
| Magnification | Ocular Lens | Total Magnification | |
| Scanning | 4x | 10x | 40x |
| Low Power | 10x | 10x | 100x |
| High Power | 40x | 10x | 400x |
| Oil Immersion | 100x | 10x | 1000x |
1. Plug your microscope into the power supply and switch on the illuminator.
2. Always start with the stage as low as possible and using scanning objective (4x). Odds are, you will be able to see something on this setting (sometimes it’s only a color). Use the coarse knob to focus: the image may be small at this magnification, but you won't be able to find it on the higher powers without this first step. Move the mechanical stage until your focused image is also centered.
3. Once you've focused using the scanning objective, switch to the low power objective (10x). Use the coarse knob to refocus and move the mechanical stage to re-center your image. Again, if you haven't focused on this level, you will not be able to move to the next level.
4. Now switch to the high power objective (40x). At this point, ONLY use the fine adjustment knob to focus specimens.
5. If the specimen is too light or too dark, try adjusting the diaphragm.
1. Store microscope with the scanning objective in place and the stage in its lowest position.
2. Wrap cords around the microscope.
3. Replace slides to original slide tray.
A large part of the learning process of microscopy is getting used to the orientation of images viewed through the oculars as opposed to with the naked eye. A common mistake is moving the mechanical stage the wrong way to find the specimen. This procedure is merely practice designed to make new users more comfortable with using the microscope.
- Compound microscope
- Microscope slide with the letter “e”
This portion of the procedure is another practice to demonstrate depth perception. Many new microscope users find it difficult to conceive that the specimen on the slide is in three dimensions. As the stage is moved up and down, different threads will be in focus.
- Compound microscope
- Microscope slide with 3 threads
- Compound microscope
- Microscope slide
- Coverslip
- Transfer pipette
- Pond water sample
The total magnification of a high-power objective lens combined with a 10x eyepiece is equal to 400x magnification, giving you a very detailed picture of the specimen in your slide.
What will be the total magnification of the ocular lens is 10x and the objective lens is 10x quizlet?
total magnification of both lenses, the objective lens X ocular lens. The ocular eyepiece usually magnifies the image 10X, and the objectives magnify the image 4X, 10X, 40X and 100X. For example, when using the 40X objective and a 10X ocular, the total magnification would be: 4010=400.
What is the total power of a microscope if the ocular lens is 10x and the objective is 40x?
Each objective lens has a different magnification. Multiply the magnification of the eyepiece by the magnification of the objective lens to produce total magnification. For example, a 10X ocular lens and a 40X objective lens will produce a total magnification of 400X (10 x 40 = 400).
What is the total magnification achieved using a 10x objective lens with a 10x eyepiece lens?
The total magnification achieved using a 10× objective lens with a 10× eyepiece lens is 20×. You have successfully focused on an object with the 10x and 40x objective lenses.
What is the total magnification if the ocular lens is 10X and the objective lens is 100X?
Total magnification = 10 X 10 = 100X (this means that the image being viewed will appear to be 100 times its actual size).
What is the total magnification of 10X ocular and 100X oil immersion objective?
1000X
The better the resolving power of the microscope, the closer together two objects can be and still be seen as separate….Terms and Definitions.
| For example: | low power: | (10X)(10X) = 100X |
| high dry: | (40X)(10X) = 400X | |
| oil immersion: | (100X)(10X) = 1000X |
What is the total magnification if the magnification of the eyepiece is 10X and the magnification of the objective lens is 40x?
400x magnification
A microscope’s total magnification is a combination of the eyepieces and the objective lens. For example, a biological microscope with 10x eyepieces and a 40x objective has 400x magnification.
What is a 10x ocular lens?
We all know that 10X means that the objective lens has an effective magnification of ten times life size and when combined in the compound with a 10X ocular lens will give a final magnification of 100X (10 X 10).
How do you calculate total magnification?
To get the total magnification take the power of the objective (4X, 10X, 40x) and multiply by the power of the eyepiece, usually 10X.
What is the total magnification at 10x and 100x?
| Scanning | 4x | 40x |
| Low Power | 10x | 100x |
| High Power | 40x | 400x |
| Oil Immersion | 100x | 1000x |
What is the total magnification when using the 10x ocular lens?
So for 10X objective and 10X ocular, Total magnification = 10 X 10 = 100X (this means that the image being viewed will appear to be 100 times its actual size). Read, more on it here. Also know, what is the total magnification when using a 10x ocular lens and a 40x objective lens?
How to calculate the total magnification of an eyepiece?
To calculate total magnification, find the magnification of both the eyepiece and the objective lenses. The common ocular magnifies ten times, marked as 10x. The standard objective lenses magnify 4x, 10x and 40x.
How many times can you magnify a microscope?
The common ocular magnifies ten times, marked as 10x. The standard objective lenses magnify 4x, 10x and 40x. If the microscope has a fourth objective lens, the magnification will most likely be 100x.
Where are the objective lenses on a microscope?
The ocular or eyepiece is found at the top of the body tube. The objective lens points down toward the object to be magnified. Most microscopes have three or four objective lenses mounted on a rotating nosepiece. Rotating the nosepiece lets the viewer change the magnification.