Do you think the addition of glucose carrier will affect the transport of sodium or potassium?

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Preparation for Physiology Assessment
Do you think the addition of glucose carriers will affect the transport of sodium?
How does glucose affect active transport?
Why is the sodium potassium pump important for the transport of glucose into the cell?
What happens to sodium and potassium during active transport?

9/8/2020PhysioEx Exercise 1 Activity 5

https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex1/act5/1/3

PhysioEx Lab Report

Exercise 1: Cell Transport Mechanisms and Permeability

Activity 5: Simulating Active Transport

Name: Shan Noorani

Date: 8 September 2020

Session ID: session-fc3b437f-5c21-88c5-491a-0acddd1f27a8

Pre-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.

Experiment Results

Predict Questions

Stop & Think Questions

The movement of sodium and potassium maintained by the Na-K pump

You correctly answered: requires energy and is against a concentration gradient.

1++

The sodium-potassium pump is classiﬁed as a(n)

You correctly answered: antiporter.

The sodium-potassium pump moves _____ sodium ions and _____ potassium ions

simultaneously.

You correctly answered: 3, 2.

Solutes that require active transport for movement might be too large to pass or might be

You correctly answered: lipid insoluble.

Predict Question 1: What do you think will result from these experimental conditions?

Your answer: No Na will be transported.

Predict Question 2: Do you think the addition of glucose carriers will aﬀect the transport of

sodium or potassium?

Your answer: No, it will not aﬀect the transport of either ion.

Why did the sodium transport stop before the transport was completed?

You correctly answered: The ATP was depleted.

Name: evelyn

Exercise 1: Cell Transport Mechanisms and Permeability: Activity 5: Simulating Active Transport Lab Report

Pre-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.

1. The movement of sodium and potassium maintained by the Na+ -K+ pump

You correctly answered: e. requires energy and is against a concentration gradient.

2. The sodium-potassium pump is classified as a(n)

You correctly answered: a. antiporter.

3. The sodium-potassium pump moves _____ sodium ions and _____ potassium ions simultaneously.

You correctly answered: b. 3, 2

4. Solutes that require active transport for movement might be too large to pass or might be

You correctly answered: a. lipid insoluble.

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Preparation for Physiology Assessment

Question	Answer
Define the active transport process	A cell using cellular energy (ATP) to move substances across its membrane
List 3 reasons why Substances moved across cell membranes by an active transport process are generally unable to pass by diffusion	It might be too large to pass through the membrane pores It might be lipid insoluble It might have to move AGAINST rather than with a concentration gradient
What are carrier proteins most commonly called?	Solute pumps
What 2 substances move into cells by solute pumps? Explain.	Amino acids & some sugars. Both of these kinds of solutes are necessary for the life of the cell, but they are lipid insoluble & too large to pass through membrane pores.
What does the sodium-potassium solute pump do?	moves Na + and K+ in opposite directions across cellular membranes. 3 Na+ ions are ejected out for ever 2 K+ ions entering the cell. Note that there is more K+ inside the cell than outside so K+tends to remain outside cell unless actively transported in.
Give an example of coupled transport.	Membrane carrier proteins that move more than 1 substance such as the Na+K+ pump
What is a symporter?	If the solutes move in the same direction, the carrier is a symporter
What is an anti porter?	If the solutes move in opposite directions, the carrier is an antiporter.
What is a uniporter?	A carrier that transports only a single solute is a uniporter.
What does ATP hydrolysis do and provide?	ATP hydrolysis provides the driving force and in many cases the substances move AGAINST concentration gradients or electrochemical gradients or both.
Resembls enzyme-substrate interaction	In one type of active transport, substances move across a membrane by combining with a carrier-protein molecule.
The movement of sodium and potassium maintained by the Na+ -K+ pump....	requires energy and is against a concentration gradient.
The sodium-potassium pump is classified as a(	antiporter
Will theddition of glucose carriers will affect the transport of sodium or potassium?	No, it will not affect the transport of either ion.
Why was the equilibrium for the solutes reached earlier?	There were more pumps for transport.
What happened when you increased the amount of ATP dispensed with the same concentration of sodium and potassium on either side of the membrane?	More ions were transported.
What was the effect of adding more Na+ -K+ pumps to the simulated cell?	Transport of the ions was faster.
Describe the effect of adding glucose carriers to the sodium and potassium transport.	There was no change in the transport rate because glucose is transported independently.
Describe the significance of using 9 mM sodium chloride inside the cell and 6 mM potassium chloride outside the cell, instead of other concentration ratios.	For every 3 Na+ transported out of cell, 2 K+ ions are transported in. The 9mM of Na+ and 6mM of K+has a 3:2 ratio.
Explain why there was no sodium transport even though ATP was present.	Because in order for Na+/K+ pumps to function, both of these ions (Na+ and K+) must be present. In this stimulation there was no potassium (K+) present, therefore the Na+/K+ pump was not able to function.
Explain why the addition of glucose carriers had no effect on sodium or potassium transport.	B/c the carriers are used to transport molecules such as glucose via facilitated diffusion with no input of energy from an external source. Na+ and K+ ions are transported actively via Na+/K+ pumps against their concentration gradient. therefore...
Do you think glucose is being actively transported or transported by facilitated diffusion in this experiment? Explain your answer.	Glucose is being transported passively down its concentration gradient by facilitated diffusion because there was no ATP present. So we can assume that glucose carriers were present to assist in the transportation of glucose molecules across the membrane.

Do you think the addition of glucose carriers will affect the transport of sodium?

Predict Question 2: Do you think the addition of glucose carriers will affect the transport of sodium or potassium? Your answer: No, it will not affect the transport of either ion.

How does glucose affect active transport?

There are two mechanisms for glucose transport across cell membranes. In the intestine and renal proximal tubule, glucose is transported against a concentration gradient by a secondary active transport mechanism in which glucose is cotransported with sodium ions.

Why is the sodium potassium pump important for the transport of glucose into the cell?

The Na-K-ATPase, also known as the sodium-potassium pump, is a transporter protein that is expressed ubiquitously in cell membranes of higher eukaryotes (20, 23). It is important for maintaining the Na⁺ gradient, which drives the apical absorption of glucose in SGLT1.

What happens to sodium and potassium during active transport?

The sodium-potassium pump transports sodium out of and potassium into the cell in a repeating cycle of conformational (shape) changes. In each cycle, three sodium ions exit the cell, while two potassium ions enter.