Sterilization indicators from Gurunanak College of Pharmacy, Nagpur Show
A sterilized surface, item, or product is one that is totally devoid of microorganisms. Sterilization processes must be strictly controlled to ensure that sterile products do not contain viable organisms.
Biological indicators
Sterilization process Species used Autoclaving at 121 degrees Clostridium sporogenes Bacillus stearothermophilus Membrane filter (0.22 micrometer) Pseudomonas diminuta. Ionizing radiations Bacillus pumilus Dry heat at 160°C Bacillus subtilis var.niger Membrane filter (0.45 micrometer) Serratia marcescens Ethylene Oxide Bacillus subtilis var.niger Physical indicatorsVarious chemical monitors are used to monitor the sterilization process. These include the following chemical monitors, which vary in properties depending on the sterilization process.Dry heatIt is possible for a Master temperature record to be prepared for a machine to serve as a reference for each batch temperature setting.Filtration methodsIn bubble point pressure testing, integrity is confirmed and pore size is determined after the process is completed.Test parameters include:
Gaseous methodsThe following steps are monitored during the gaseous sterilization process:
Moist heat
Chemical indicatorsBy sterilizing the chemical monitors, the physical or chemical properties of the chemical monitors' change.These are the chemical monitors most commonly used: Witness tubes
Royce sachet
Heat sensitive tape
Chemical dosimeter
Brown's tube
Get subject wise printable pdf documentsView Here Share Tweet Share Share What are the different types of sterilization indicators?6 ISO Types of Chemical Indicators for Steam Sterilization. Type 1: Process Indicators.. Type 2: Specific-Use Indicators.. Type 3: Single-Variable Indicators.. Type 4: Multi-Variable indicators.. Type 5: Integrating Indicators (Integrators). Type 6: Emulating Indicators (Cycle Verification Indicators). What is a Type 4 chemical indicator?The Type 4 Chemical Indicator confirms that all critical parameters of steam sterilization have been met: time, temperature, and saturation (ANSI/AAMI/ISO 11140-1:2014 ). OK® Strips feature an instantly recognizable “OK” symbol after exposure to an effective steam sterilization cycle.
What is a Type 5 chemical indicator?Type 5 Integrating Indicators:
These specialty indicators contain a steam sensitive chemical that imparts a physical change, such as movement of the chemical along a strip or a change of ink from one color to another when all critical parameters of sterilization have been met.
What is a Type 2 chemical indicator?Class 2: indicators for use in specific tests Class 2 indicators are intended for use in specific test procedures as defined in relevant sterilizer/sterilization standards. Bowie-Dick type tests are specific tests used for quipment control to evaluate the sterilizer performance.
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