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You must have the object centered before you change objectives to increase the magnification, because the field of view becomes smaller; if the object is off to the side, it may disappear when you go to higher magnification. For best viewing at high power, white light is essential. Explanation: #BRAINLIEST_BUNCH#CARRY_ON_LEARNING#HOPE_IT_HELPSMicroscope NotesThe compound microscope is a useful tool for magnifying objects up to as much as 1000 times their normal size. Using the microscope takes lots of practice. Follow the procedures below both to get the best results and to avoid damaging the equipment.
Field of ViewThe field of view is largest on the lowest power objective. When you switch to a higher power, the field of view closes in towards the center. You will see more of an object on low power. Therefore, it is best to find an object on low power, center it, and then switch to the next higher power and repeat. Depth of FocusThe depth of focus is greatest on the lowest power objective. Each time you switch to a higher power, the depth of focus is reduced. Therefore a smaller part of the specimen is in focus at higher power. Again, this makes it easier to find an object on low power, and then switch to higher power after it is in focus. A common exercise to demonstrate depth of focus involves laying three different colored threads one on top of the other. As the observer focuses down, first the top thread comes into focus, then the middle one, and finally the bottom one. On higer power objectives one may go out of focus as another comes into focus. Microscope TroubleshootingProblem #1: The image is upside down and/or backwards.
Problem #2: Everything is dark.
Problem #3: I can't find anything on low power!
Problem #4: When I moved to a higher power, everything disappeared!
Problem #5: The image is blurry on all powers.
Problem #6: The image is blurry only on a particular power.
Microscope DrawingsWhen drawing what you see under the microscope, follow the format shown below. It is important to include a figure label and a subject title above the image. The species name (and common name if there is one) and the magnification at which you were viewing the object should be written below the image. All relevant parts of the drawing should be labelled on the right side of the image using straight lines. Lines should not cross. Drawings should be done in pencil, while labels should be in pen or typed. Remember that total magnification is determined by multiplying the ocular x objective. Viewing Prepared Slides*** Don't hoard slides! You can only view one at a time, so that's all you should be holding. Return it before getting another, and if you break it, tell your instructor so that it can be properly cleaned up and replaced! ***
Making a Wet Mount (Live Prep) Slide
Further InvestigationDigital microscope for Macintosh or Windows Investigating pondwater organisms Powers of 10 (1977 version) Make your own microscope Why do you have to center the specimen before moving to the next higher objective lens?Answer and Explanation: The field of view in a microscope is highest on the lowest power objective. When one switches to higher magnification power, the field of view is more concentrated towards the center. Therefore one can see the object more clearly on lower power.
Why must you center and focus the object in the field of view under low power?The field of view is largest on the lowest power objective. When you switch to a higher power, the field of view closes in towards the center. You will see more of an object on low power. Therefore, it is best to find an object on low power, center it, and then switch to the next higher power and repeat.
Why is it important to center the object you wish to view before moving to the next higher power?Answer and Explanation: It's important to center a specimen prior to increasing microscope magnification because, the lenses focus on the center of a subject. If the subject in question isn't centered, when magnification occurs the lenses will magnify whatever is in the center of the optical frame.
What could happen to a small object seen through the ocular lens as you change from a low power to a high power objective lens?Changing from low power to high power increases the magnification of a specimen. The amount an image is magnified is equal to the magnification of the ocular lens, or eyepiece, multiplied by the magnification of the objective lens.
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